The gene.The AXL protein encoded by this gene to human UFO, tyrosine protein kinase receptor is an enzyme encoded by a member of the receptor tyrosine kinase family. It is similar to the unique structure of the region outside the association IGL FNIII iterative other receptor tyrosine kinases, and Axl protein. It transmits the signal from the cytoplasm extracellular matrix by growth factors such as vitamin K-dependent protein growth arrest-specific gene 6 binds. This is included in the stimulation of cell proliferation. This receptor can mediate cell aggregation by homophilic binding.
As well as being related oncogene in chronic myelogenous leukemia, AXL is associated with melanoma and colon cancer. It is located near the oncogene BCL3 is 19q13.1-q13.2. Accelerator gene is conserved in evolution between vertebrate species. The gene to interact with TENC1 have been shown to transfer variants.AXL receptor tyrosine kinase that is spliced alternatively two different. AXL is a regulator and transitional cell induction to the major mesenchymal transition of the survival rate of patients with breast cancer.
Tyrosine phosphorylation and tyrosine kinase, may include a growth factor receptor and oncogenes several important in the transmission of signals differentiation and proliferation known. Interestingly, such as red blood cells and platelets, differentiated cell types, some contain a large amount of tyrosine phosphorylation. We analyzed the expression tyrosine kinase megakaryoblastoid erythroid differentiation capacity and performance of both the chronic myelogenous leukemia line K-562. Analysis of cDNA clones 359 PCR amplification, tyrosine kinase-associated sequences of 14 types were identified. (JTK4 and JTK2) two branches is JTK5, JTK11 JTK14 branch members and abnormal fibroblast growth factor receptor gene, belong to the family of receptor tyrosine kinases, closely tyrosine kinase known all It may have a relationship Na. Each of the various genes of these equations has a pattern characteristic of its own in human tumor cell lines and several other K-562 cells. In addition, JTK14 mRNA and JTK11 induced differentiation megakaryoblastoid K-562 cells. The tyrosine kinase of these, may play a physiological role of platelets and differentiation of megakaryocytes blasts.
Phosphorylation recognition module two different, SHC adapter protein, less quality ShcA to, N-terminal phospho and 2 domain C-terminal Src homology – has a binding domain itself in response to multiple signals of extracellular , it is phosphorylated at tyrosine. The coupling inductor to the SH2 domain of the Grb2, phosphorylation quality ShcA human Tyr-317 in the central region is involved in activation of the Ras pathway results. Related genes of two SHC have been identified in the mouse. shcB is related to a human shcC SCK, are not found in other organisms still closely. It has a C-terminal SH2 domain CH1 region and the N-terminal PTB domain binding site and the putative protein Grb2 in ShcC is expected. Phosphorylated tyrosine-containing peptides that bind to the SH2 domain ShcC ShcB, rather than receptor specificity that is associated with call me, different from the domain of quality ShcA SH2. Autophosphorylation the epidermal growth factor receptor and ShcC PTB domain nerve growth factor associated with in vitro particular. These results, domain, indicating the PTB and the function ShcC SH2. Unlike SHCA to be widely expressed, and proteins shcC RNA is expressed in the adult brain, mainly. These results, ShcC, suggesting that it may mediate tyrosine kinases of the nervous system, signaling by receptors for neurotrophins.
Proliferation of mesangial cells, a feature of glomerular disease, understanding its regulatory mechanism is important clinically. To stimulate mesangial cell proliferation products through binding to cell surface receptors on the accelerator in vitro from the growth arrest-specific gene 6 previously indicated. We also human IgG 1 and warfarin have shown that the extracellular domain of the accelerator associated with the Fc portion of inhibiting mesangial cell proliferation through interference Gas6/Axl time (accelerator-FC) in vitro. In this study, therefore, we test in the role of the in vivo of the accelerator and GAS6 in experimental models of induced mesangial proliferative glomerulonephritis by injection of anti-Thy1.1 antibody. THY1 GN, a glomerular expression and accelerator GAS6, and significantly higher in parallel with the development of mesangial cell proliferation. To inhibit the proliferation of mesangial cells, daily injections of accelerator-Fc or administration of warfarin, to remove the protein and mRNA induction of platelet-derived growth factor-B in THY1 GN. In addition, anti-proliferative effect of warfarin is achieved at concentrations lower than the clinical routine use. These results indicate that it plays an important role in mesangial cell proliferation in in vivo, methods Gas6/Axl may be a therapeutic target potentially important for the treatment of kidney disease.
We, and a member of the vitamin K-dependent protein family growth arrest-specific gene 6 of plural human uterine, ovarian endometrial endometrium and (GAS6), the receptor tyrosine kinase using the RT, the expression of GAS6, immunohistochemistry, and Southern blot analysis of protein product and empty the accelerator-PCR is shown. GAS6, accelerator, Sky mRNA was detected in all samples tested. There was no significant difference between the level of Sky mRNA in the plural of endometriosis endometrium and normal uterus in there, but the level of GAS6 mRNA is, of normal endometrium in the plural of endometriosis endometrium and accelerator It was significantly higher than in the plural form. MRNA levels, these showed no significant changes during the menstrual cycle. In the immunohistochemical study, GAS6, Sky and Axel was detected in stromal cells and endometrial cells in a test sample of all. In this study, it indicates that ligand and receptor tyrosine kinases in overexpression and plural form of endometriosis endometrium of GAS6 the accelerator, the co-expression of GAS6 in the plural of the endometrium endometrium of the uterus and ovaries of normal . Incidentally, the accelerator and GAS6 signaling stimulates plural form of the abnormal endometriosis endometrium, it is assumed that plausible in relation to the growth potential.