The (ephrin receptor type 3), EPH receptor A3 is a protein encoded by a gene EPHA3 in humans. This gene belongs to the ephrin receptor subfamily of protein tyrosine kinase. In the central nervous system, EPH-related receptors EPH is involved in mediating a particular developmental event. Usually, EPH subfamily receptors, have an extracellular region containing two fibronectin type III repeats and cysteine-rich domain and kinase domain of the single. Are divided into two groups based on the similarity of their affinity for the ligand ephrin binding and ephrin-AB extracellular domain sequence of the ephrin receptor. This gene encodes a protein that binds to ephrin ligand. Transcript variants that are alternatively spliced ​​two have been described for this gene.

This gene belongs to the ephrin receptor subfamily of protein tyrosine kinase. In the central nervous system, EPH-related receptors EPH is involved in mediating a particular developmental event. Usually, EPH subfamily receptors, have an extracellular region containing two fibronectin type III repeats and cysteine-rich domain and kinase domain of the single. Are divided into two groups based on the similarity of their affinity for the ligand ephrin binding and ephrin-AB extracellular domain sequence of the ephrin receptor. This gene encodes a protein that binds to ephrin ligand. Transcript variants that are alternatively spliced ​​two have been described for this gene.

We have described the molecular cloning of the receptor tyrosine kinase cell lines derived from the case of Hitopure B cell leukemia (LK63). Monoclonal antibodies raised against LK63 earlier (I IIA4), recognizing molecules 135 kDa glycosylated cell surface showing tyrosine kinase activity in vitro and (NEC) we have shown. Purification by IIIA4 antibody column, HEK protein was obtained internal amino acid sequence and N-terminus of both. Oligonucleotide 51 mer degenerate based on internal amino acid sequences were used to screen a GT10 cDNA library prepared LK63 Lambda low-stringency hybridization conditions. Clones either 2.5 kg base (KB) is isolated and characterized, and was used to rescreen the library under stringent hybridization conditions and more. The isolated clone of 4.5 KB NEC containing the coding region of the entire, complete sequence of the DNA is determined. 4,5 – The KB introduction, I was transferred to COS cells and CDM8 subcloned into the expression vector. Make sure that you recognize IIIA4 expressed protein product of the HEK cDNA clone of the same antibody and antigen as this, COS cells transfected with constructs IIIA4 antibody specific sense HEK/CDM8 stained. Analysis of the DNA sequence, members of the EPH / ELK family of receptor tyrosine kinases newly discovered HEK. Northern blot analysis of several cell lines showed expression of transcripts of 5.5 ~ 6.0-B in HSB-2 and T cell lines JM and HEK LK63. The Southern blot analysis of DNA from LK63 assumed HEK gene was also amplified, be relocated to the tumor LK63.

This gene belongs to the ephrin receptor subfamily of protein tyrosine kinase. In the central nervous system, EPH-related receptors EPH is involved in mediating a particular developmental event. The receptor in EPH Subfamily has an extracellular region containing two fibronectin type III repeats and a cysteine-rich domain and the kinase domain of a single, typically. Are divided into two groups based on the similarity of their affinity for the ligand ephrin binding and ephrin-AB extracellular domain sequence of the ephrin receptor. The gene encoding this Protein that binds to ephrin ligand. Transcript variants that are alternatively spliced ​​two have been described for this gene.

In the contact-dependent bidirectional signaling device to adjacent cells, and as a result, receptor tyrosine kinase that binds to the membrane-bound ephrin ligand family random present in the cell adjacent to each other. Downstream between signaling pathways ligand ephrin reverse signaling is called downstream signaling the first signaling path of the receptor. Ephrin ligands are very promiscuous in order to bind EFNA5 priority. Upon activation that regulate cell migration cell EFNA5 cell adhesion, and organization of the cytoskeleton. Via activation of EFNA1 that it plays an important role in the migration and differentiation of cardiac cells, to regulate the formation of the septum and the atrioventricular groove during development probably. And involved in retinal photoreceptor neurons cover mapping. You can rather than separation, to control the management of motor and sensory axons during the development of neural

Four hetero ligand binding. I consists of ephrin receptor dimer and tetramer hetero. Oligomerization, it is necessary to induce a biological response probably. I want to form a complex three 10 EFNA5-EPHA3-ADAM EFNA5 extracellular domain shedding mediation of ADAM 10, which govern the function and EFNA5-EPHA3 complex sound. You interact with NCK1 (via SH2 domain) mediate (C similar) signaling EFNA5-EPHA3. I interact
And (phosphorylated) PTPN1; EPHA3 to dephosphorylation, you can adjust the function and trafficking. (Phosphorus) interact with CRK; Through the GTP-ase activation of intracellular localization of RhoA, EFNA5-EPHA3 mediated signaling: The type I membrane protein cell membrane, single-pass: 1 isoforms

It is applied to identify the (GBM) considerable effort functions therapeutic interest in glioblastoma for suspension growth of aggressive cancers. In GBM often, in particular, we show the receptor tyrosine kinase EphA3 that is over-expressed in mesenchymal subtype most aggressive. I suspect Importantly, EphA3 is expressed in glioma tumor initiating cell population is very essential to the maintenance of tumor cells in the differentiated state by modulation of mitogen-activated protein kinase signaling. Or depletion EphA3 knockdown EphA3 positive tumor cells, reduced the tumorigenicity of the degree comparable to treatment with monoclonal antibodies EphA3-specific therapeutic radiolabeled. These results, define the EphA3 as functional target receptor in GBM.