By recombinant techniques and EphA9 polynucleotides and polypeptides, methods for producing such polypeptides are disclosed. In addition, how to use the polynucleotide and polypeptide EphA9 in diagnostic assays is disclosed.

Protein tyrosine kinases (TK) receptors can play an important role in various aspects of tumor formation and normal development is known. Germ cells of colonization before their gonads during embryogenesis, is called primary germ cells (primordial germ cells). Identifying the expressed TK gene in chicken primordial germ cells, we extracted () RNA poly purified them from blood cells-old embryos 2.5 days, in reverse (RT-PCR) amplification transcription-polymerase chain reaction and TK supply gene-specific primers. As a result, we identified non-receptor 6 TK gene and gene 13 TK receptors. New printed, therefore, in addition to the RNA sequence (cDNA), the length of DNA, one of the receptor TK gene is removed from the rapid method of cloning cDNA ends. Sequence analysis of the DNA showed that it encodes the Eph family of which it is the receptor TK novel. Is 63.0% identical to the human EphA1, therefore, deduced amino acid sequence of the TK, defined as EphA9 a new TK we. The kidney, lung, testis, and ephA9 RNA transcripts were present in the thymus is not revealed, Northern blot hybridization is the liver spleen, and brain. The expression of a fusion protein containing the intracellular domain of EphA9 in bacterial cells, this field indicates that it is a TK enzyme activity. Is a phosphorylated tyrosine EphA9 seed production on a COS-1 cells that were transfected with the expression vector. These data indicate that the EphA9 is active as TK, in a variety of organs of chicken, and plays its biological role.

“Functional genomics”, ie, to accompany the biology of gene-based or high-performance genome, currently, the process of drug discovery has undergone a revolution fundamental it. As a means for therapeutic target based on “positional cloning” rapidly and to identify genes and gene products, such as replacement of previous approaches, this approach. It is a genetic disease or identified biological function, and then based on the position of the gene map, phenotype is traced back to the responsible gene.

Currently, functional genomics relies heavily on bioinformatics tools and different tech DNA to identify gene sequences of potential interest in molecular biology databases available lot. Continuing need for the identification and characterization their related polypeptides / proteins and further genes as drug targets exist.

Protein tyrosine kinases (TK) receptors can play an important role in various aspects of tumor formation and normal development is known. Germ cells of colonization before their gonads during embryogenesis, is called primary germ cells (primordial germ cells). Identifying the expressed TK gene in chicken primordial germ cells, we extracted () RNA poly purified them from blood cells-old embryos 2.5 days, in reverse (RT-PCR) amplification transcription-polymerase chain reaction and TK supply gene-specific primers. As a result, we identified non-receptor 6 TK gene and gene 13 TK receptors. New printed, therefore, in addition to the RNA sequence (cDNA), the length of DNA, one of the receptor TK gene is removed from the rapid method of cloning cDNA ends. Sequence analysis of the DNA showed that it encodes the Eph family of which it is the receptor TK novel. Is 63.0% identical to the human EphA1, therefore, deduced amino acid sequence of the TK, defined as EphA9 a new TK we. The kidney, lung, testis, and ephA9 RNA transcripts were present in the thymus is not revealed, Northern blot hybridization is the liver spleen, and brain. The expression of a fusion protein containing the intracellular domain of EphA9 in bacterial cells, this field indicates that it is a TK enzyme activity. Is a phosphorylated tyrosine EphA9 seed production on a COS-1 cells that were transfected with the expression vector. These data, as organs such as chicken active TK such, the role of EphA9 play its biological was suggested.

The monoclonal mouse for EphA9. EphA8: EPH receptor A8. This gene encodes a member of the ephrin receptor subfamily of protein tyrosine kinases. In the central nervous system, EPH-related receptors EPH is involved in mediating a particular developmental event. Usually, EPH subfamily receptors, have an extracellular region containing two fibronectin type III repeats and cysteine-rich domain and kinase domain of the single. Are divided into two groups based on the similarity of their affinity for the ligand ephrin binding and ephrin-AB extracellular domain sequence of the ephrin receptor. Protein is encoded by a gene that functions as a receptor for A5 and A2, A3 ephrins, play a role in short-range contact-mediated axonal guidance during development of the mammalian nervous system.

A polypeptide comprising a polypeptide sequence having at least 99% identity to the polypeptide sequence of 1, SEQ ID NO: the isolated polypeptide, poly encoded by a polynucleotide comprising the sequence of SEQ ID NO The two selected from the group consisting of peptides, at 42 ℃ that the coding in a solution containing 1 ° under a stringent comprising overnight incubation the polypeptide C: 50% formamide, 5 × SSC (150 Wash the filter with 0.1 × SSC at 65 ℃ by a polynucleotide that hybridizes to the complement of SEQ ID NO: 1 NaCl, mM, trisodium citrate 15mM), at 50mM, sodium phosphate, 5 × followed Denhardt’s solution, 10% dextran sulfate, and DNA fragmentation of denatured salmon sperm of 20 micrograms / ml, ° C, the polypeptide, the polypeptide sequence of SEQ ID NO activity and has a ligand binding ephrinA 1:2, polypeptides claims encoded by a polynucleotide that hybridizes to the complement of SEQ ID NO 1 under a stringent comprising overnight incubation at 42 ℃: polypeptide having at least 99% identity and 50% formamide, 5 × SSC (NaCl of 150 mM, trisodium citrate 15mM), 50mM sodium phosphate, 5 × solution Denhardt’s, 10% dextran sulfate, 20 micrograms /: ° C in a solution containing salmon sperm used to clean the filter with 0.1 × SSC at 65 ℃ denaturation of DNA shearing ml, ° C, wherein the polypeptide has a ligand-binding activity ephrinA.